# WIA-AGRI-034: Phase 1 - Data Format Specification **Standard ID:** WIA-AGRI-034 **Title:** Single Cell Protein Production Data Format **Version:** 1.0 **Status:** Active **Last Updated:** 2025-01-15 --- ## 1. Overview This specification defines the standardized data format for Single Cell Protein (SCP) production systems, enabling interoperability between different fermentation platforms, quality control systems, and supply chain stakeholders. ### 1.1 Scope This standard covers: - Microbial strain documentation - Fermentation process parameters - Quality and safety metrics - Production and efficiency data - Environmental impact measurements ### 1.2 Objectives - **Interoperability**: Enable seamless data exchange between SCP production systems - **Traceability**: Maintain complete audit trails from strain to final product - **Quality Assurance**: Standardize quality metrics and testing protocols - **Optimization**: Facilitate AI/ML-driven process optimization - **Sustainability**: Track environmental impact and resource efficiency --- ## 2. Core Data Structures ### 2.1 Batch Record ```json { "batch_id": "string (required, unique)", "version": "1.0", "protocol": "WIA-AGRI-034", "timestamp": "ISO8601 datetime", "facility": { "id": "string", "name": "string", "location": { "country": "string", "region": "string", "coordinates": { "latitude": "number", "longitude": "number" } }, "certifications": ["string"] }, "strain": { "id": "string (required)", "taxonomy": { "domain": "Bacteria | Eukarya", "kingdom": "string", "phylum": "string", "class": "string", "order": "string", "family": "string", "genus": "string", "species": "string" }, "strain_name": "string", "genetic_modifications": [ { "type": "CRISPR | traditional | none", "target_gene": "string", "modification": "string", "purpose": "string" } ], "safety_classification": "GRAS | approved | experimental", "growth_characteristics": { "optimal_temperature_C": "number", "temperature_range_C": { "min": "number", "max": "number" }, "optimal_pH": "number", "pH_range": { "min": "number", "max": "number" }, "generation_time_hours": "number", "oxygen_requirement": "aerobic | anaerobic | facultative" } } } ``` ### 2.2 Fermentation Parameters ```json { "fermentation": { "batch_id": "string (FK)", "start_time": "ISO8601 datetime", "end_time": "ISO8601 datetime", "duration_hours": "number", "reactor_type": "stirred_tank | airlift | bubble_column | fed_batch", "working_volume_L": "number", "substrate": { "primary_source": "methanol | methane | glucose | molasses | agricultural_waste | co2_h2", "concentration_g_L": "number", "feed_rate_g_h": "number (for fed-batch)", "total_consumed_kg": "number" }, "process_parameters": { "temperature": { "setpoint_C": "number", "actual_C": "number", "variance_C": "number", "control_method": "PID | on_off | cascade" }, "pH": { "setpoint": "number", "actual": "number", "control_agent_acid": "string", "control_agent_base": "string", "consumption_L": "number" }, "dissolved_oxygen": { "setpoint_percent": "number", "actual_percent": "number", "air_flow_rate_vvm": "number", "oxygen_enrichment_percent": "number" }, "agitation": { "speed_rpm": "number", "impeller_type": "rushton | pitched_blade | marine", "power_input_w_m3": "number" }, "pressure": { "headspace_kPa": "number", "control_method": "string" } }, "nutrient_feed": { "nitrogen_source": "ammonium_sulfate | ammonium_hydroxide | urea | nitrate", "nitrogen_g_L": "number", "phosphorus_source": "string", "phosphorus_mg_L": "number", "potassium_mg_L": "number", "magnesium_mg_L": "number", "trace_elements": [ { "element": "Fe | Mn | Zn | Cu | Co | Mo | B", "concentration_mg_L": "number" } ], "vitamins": [ { "name": "B1 | B12 | biotin | pantothenic_acid", "concentration_mg_L": "number" } ] } } } ``` ### 2.3 Real-time Sensor Data ```json { "sensor_data": { "batch_id": "string (FK)", "timestamp": "ISO8601 datetime", "measurements": [ { "sensor_id": "string", "parameter": "temperature | pH | DO | biomass | substrate | product", "value": "number", "unit": "string", "quality": "good | fair | poor", "calibration_date": "ISO8601 date" } ], "biomass": { "optical_density_600nm": "number", "dry_cell_weight_g_L": "number", "viable_cell_count_cfu_mL": "number" }, "metabolites": { "substrate_remaining_g_L": "number", "protein_content_g_L": "number", "byproducts": [ { "compound": "string", "concentration_g_L": "number" } ] } } } ``` ### 2.4 Quality Metrics ```json { "quality_analysis": { "batch_id": "string (FK)", "analysis_date": "ISO8601 datetime", "laboratory": "string", "accreditation": "ISO17025 | other", "composition": { "protein_content": { "value_percent": "number (required)", "method": "Kjeldahl | Dumas | other", "nitrogen_to_protein_factor": "number" }, "moisture_percent": "number", "ash_percent": "number", "fat_percent": "number", "fiber_percent": "number", "carbohydrate_percent": "number" }, "amino_acid_profile": { "method": "HPLC | ion_exchange", "essential_amino_acids": { "histidine_g_100g": "number", "isoleucine_g_100g": "number", "leucine_g_100g": "number", "lysine_g_100g": "number", "methionine_g_100g": "number", "phenylalanine_g_100g": "number", "threonine_g_100g": "number", "tryptophan_g_100g": "number", "valine_g_100g": "number" }, "amino_acid_score": "number (0-1)", "limiting_amino_acid": "string" }, "digestibility": { "in_vitro_percent": "number", "in_vivo_percent": "number", "pdcaas": "number (Protein Digestibility Corrected Amino Acid Score)", "diaas": "number (Digestible Indispensable Amino Acid Score)" }, "safety_testing": { "microbial_contamination": { "total_plate_count_cfu_g": "number", "yeast_mold_cfu_g": "number", "coliforms_cfu_g": "number", "salmonella": "detected | not_detected", "e_coli": "detected | not_detected" }, "heavy_metals": { "lead_mg_kg": "number", "cadmium_mg_kg": "number", "mercury_mg_kg": "number", "arsenic_mg_kg": "number" }, "mycotoxins": { "aflatoxin_b1_ug_kg": "number", "ochratoxin_a_ug_kg": "number", "deoxynivalenol_ug_kg": "number" }, "allergens": ["string"], "nucleic_acid_content_percent": "number" }, "nutritional_value": { "energy_kcal_100g": "number", "bioavailable_protein_g_100g": "number", "vitamins": [ { "name": "string", "amount": "number", "unit": "mg | ug" } ], "minerals": [ { "name": "string", "amount_mg_100g": "number" } ] }, "overall_grade": "A+ | A | B | C | D | F", "certification_status": "organic | non_gmo | kosher | halal | vegan" } } ``` ### 2.5 Production Metrics ```json { "production_metrics": { "batch_id": "string (FK)", "yield": { "biomass_yield_g_L": "number", "protein_yield_g_L": "number", "volumetric_productivity_g_L_h": "number", "specific_productivity_g_g_h": "number", "yield_coefficient_g_biomass_g_substrate": "number" }, "efficiency": { "substrate_conversion_percent": "number", "carbon_conversion_percent": "number", "nitrogen_utilization_percent": "number" }, "economics": { "substrate_cost_usd_kg": "number", "energy_cost_usd_kg": "number", "labor_cost_usd_kg": "number", "total_production_cost_usd_kg": "number", "estimated_market_price_usd_kg": "number", "profit_margin_percent": "number" }, "environmental_impact": { "carbon_footprint_kg_co2eq_kg_protein": "number", "water_consumption_L_kg_protein": "number", "land_use_m2_kg_protein": "number", "energy_consumption_kwh_kg_protein": "number", "waste_generated_kg_kg_protein": "number", "circular_economy_score": "number (0-100)" } } } ``` --- ## 3. Data Validation Rules ### 3.1 Required Fields - `batch_id`: Must be unique across all records - `timestamp`: Must be valid ISO8601 format - `strain.id`: Must reference valid strain database entry - `quality_analysis.composition.protein_content.value_percent`: Must be present and >0 ### 3.2 Value Ranges ```yaml temperature_C: [15, 80] pH: [2.0, 11.0] dissolved_oxygen_percent: [0, 200] protein_content_percent: [30, 95] amino_acid_score: [0.0, 1.0] ``` ### 3.3 Data Quality Indicators - `timestamp_accuracy`: ±1 second - `sensor_calibration_interval`: Maximum 30 days - `quality_analysis_completion`: Within 48 hours of batch completion --- ## 4. File Formats ### 4.1 Supported Formats - **JSON**: Primary format for API exchange - **JSON-LD**: For semantic web integration - **CSV**: For bulk data export/import - **Parquet**: For big data analytics - **Protocol Buffers**: For high-performance systems ### 4.2 Encoding - Character encoding: UTF-8 - Date/time: ISO8601 with timezone - Numbers: IEEE 754 double precision - Coordinates: WGS84 decimal degrees --- ## 5. Metadata Standards ### 5.1 Dataset Metadata ```json { "metadata": { "standard": "WIA-AGRI-034", "version": "1.0", "created": "ISO8601 datetime", "modified": "ISO8601 datetime", "creator": { "organization": "string", "contact": "string" }, "license": "CC-BY-4.0 | proprietary | other", "language": "en | ko | other", "keywords": ["single cell protein", "fermentation", "alternative protein"], "related_standards": ["WIA-AGRI-001", "WIA-AGRI-002"] } } ``` --- ## 6. Compliance Requirements ### 6.1 Regulatory Compliance - FDA GRAS notification requirements - EU Novel Food Regulation (EU) 2015/2283 - Codex Alimentarius standards - National food safety regulations ### 6.2 Data Privacy - GDPR compliance for EU operations - Data anonymization for research sharing - Access control and audit logging --- ## 7. Version History | Version | Date | Changes | |---------|------|---------| | 1.0 | 2025-01-15 | Initial release | --- **弘益人間 (홍익인간) - Benefit All Humanity** © 2025 SmileStory Inc. / WIA